An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies
We present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin.Our protocol enabled the isolation of a consistently high number of highly viable skin cells from equi-jec 6 small freshly dissociated punch skin biopsies, which